Gene Expression Analysis by Multiplex Single-Cell RT-PCR - Sorbonne Université
Book Sections Year : 2019

Gene Expression Analysis by Multiplex Single-Cell RT-PCR

Bruno Cauli
Bertrand Lambolez

Abstract

Brain circuit assemblies comprise different cellular subpopulations that exhibit morphological, electrophysiological, and molecular diversity. Here we describe a protocol which, combined with whole-cell patchclamp recording and morphological reconstruction, allows the transcriptomic analysis of the recorded cell. This protocol provides recipes on how to detect simultaneously the expression of 24 genes/ markers at the single-cell level using polymerase chain reaction (PCR), how to design gene-specific probes, and how to validate them. This technique provides multiplexed expression data that cannot be easily obtained by other approaches such as immunological co-labeling.
Fichier principal
Vignette du fichier
429758_1_En_10_Chapter_Author.pdf (373.67 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03044302 , version 1 (07-12-2020)

Identifiers

Cite

Ludovic Tricoire, Bruno Cauli, Bertrand Lambolez. Gene Expression Analysis by Multiplex Single-Cell RT-PCR. Glutamate Receptors, pp.139-154, 2019, ⟨10.1007/978-1-4939-9077-1_10⟩. ⟨hal-03044302⟩
32 View
278 Download

Altmetric

Share

More