Release of infectious virus and cytokines in nasopharyngeal swabs from individuals infected with non-alpha or alpha SARS-CoV-2 variants: an observational retrospective study - Sorbonne Université Access content directly
Journal Articles EBioMedicine Year : 2021

Release of infectious virus and cytokines in nasopharyngeal swabs from individuals infected with non-alpha or alpha SARS-CoV-2 variants: an observational retrospective study

Blandine Monel
Isabelle Staropoli
Pedro Goncalves
Galdric Orvoen
Maxime Wack
Sandrine Imbeaud
James P. Di Santo
Hélène Péré

Abstract

Background The dynamics of SARS-CoV-2 alpha variant shedding and immune responses at the nasal mucosa remain poorly characterised. Methods We measured infectious viral release, antibodies and cytokines in 426 PCR+ nasopharyngeal swabs from individuals harboring non-alpha or alpha variants. Findings With both lineages, viral titers were variable, ranging from 0 to >106 infectious units. Rapid antigenic diagnostic tests were positive in 94% of samples with infectious virus. 68 % of individuals carried infectious virus within two days after onset of symptoms. This proportion decreased overtime. Viable virus was detected up to 14 days. Samples containing anti-spike IgG or IgA did not generally harbor infectious virus. Ct values were slightly but not significantly lower with alpha. This variant was characterized by a fast decrease of infectivity overtime and a marked release of 13 cytokines (including IFN-b, IP-10 and IL-10). Interpretation The alpha variant displays modified viral decay and cytokine profiles at the nasopharyngeal mucosae during symptomatic infection.

Domains

Virology
Fichier principal
Vignette du fichier
PIIS2352396421004308.pdf (3.71 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03408666 , version 1 (29-10-2021)

Licence

Attribution - NonCommercial - NoDerivatives

Identifiers

Cite

Blandine Monel, Delphine Planas, Ludivine Grzelak, Nikaïa Smith, Nicolas Robillard, et al.. Release of infectious virus and cytokines in nasopharyngeal swabs from individuals infected with non-alpha or alpha SARS-CoV-2 variants: an observational retrospective study. EBioMedicine, 2021, 73, pp.103637. ⟨10.1016/j.ebiom.2021.103637⟩. ⟨hal-03408666⟩
79 View
40 Download

Altmetric

Share

Gmail Facebook X LinkedIn More