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Analyse structurale des carraghénanes par hydrolyse enzymatique

Abstract : Carrageenans are linear sulphated polysaccharides occurring as cell wall constituents in red algae and are used as texturing agents in the food industry. The backbone of the polymer is composed of altemating α( 1→3) and ß( 1→4)-linked D-galactopyranose units. The structure of the repeating units differs in the degree and position of sulphate esterification. 3,6-anhydro-ct- D-galactopyranose units can also replace the α(1→3)-D-galactopyranose units. Structural studies of carrageenan have been conducted using carrageenases which are glycoside hydroiass produced by marine bacteria. They degrade specifieally carrageenans by cleaving their ß-(1→4) bounds. The oligo-carrageenans obtained have been analysed by liquid chromatography and characterised by mass spectrometry and NMR. The structure and the proportion of oligo-carrageeenans formed are representative of the polymer structure, These studies were performed on the heterogeneous structure of λ-, κ/ι- et κ/μ-carrageenans with λ-, κ- et ι-carrageenases. Characterisation of hybrid oligosaccharides within the hydrolytic products has confirmed the heterogeneity of these structures. Different organisation models (block, random) of the repetitive units within these heterogeneous chains have been proposed. Carrageenases should also be useful in the analysis of other more complex earrageenan structures and can be used directly on the algal ceil wall.
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Submitted on : Thursday, February 12, 2015 - 11:28:16 AM
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  • HAL Id : tel-01115975, version 1


Marion Guibet. Analyse structurale des carraghénanes par hydrolyse enzymatique. Biochimie, Biologie Moléculaire. Université de Bretagne Occidentale, 2007. Français. ⟨tel-01115975⟩



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